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Water Immersion Objective (수침 대물렌즈)

What is water immersion technology?

What is water immersion technology

Water immersion, in terms of high-content imaging, is the technology that automatically and consistently places a layer of water between the objective (magnification) and the sample.

Many cell imaging systems are designed to have air between the objective and the sample. When using air objectives for 3D imaging, a significant portion of the fluorescent light that is emitted from a sample is refracted away from the objective and becomes lost. This reduces the signal that is collected from the sample, and causes the sample to appear distorted.

Oil objectives have been the gold standard for 3D confocal imaging, because they collect more light from the sample, which increases the signal intensity and enhances the image resolution. However, oil objectives are still problematic, as oil can be difficult to handle in an automated fashion and these objectives can also cause some distortion when imaging large 3D samples.

ImageXpress Micro Confocal 시스템용 수침 대물렌즈

Scientists often experience difficulties screening complex models and obtaining better data quality. With water immersion objectives on the ImageXpress® Micro Confocal High-Content Imaging System, they can increase signal up to four times, improve z-resolution, and decrease optical aberrations for sharper, crisper images.

To address 3D image analysis workflow challenges, our MetaXpress® 3D analysis software module enables scientists to generate greater phenotypic data within a single interface without compromising throughput or data quality, giving scientists confidence in their discoveries.

3D assay using water immersion objective

Increased average intensity in 3D assay using water immersion objective. Nuclei of spheroids at 50 ms exposure, maximum projection using confocal imaging.

Water immersion cell count

^†고객이 제공한 샘플을 사용하여 개발 중 데이터 및 이미지를 획득했습니다. 결과에는 차이가 있을 수 있습니다.

Gain the sensitivity to capture more phenotypic data at greater depths

Water immersion objectives can offer signal increases of up to 4X^† which can help scientists see deeper into 3D and thick tissue samples at lower exposure times.

최대 4배 더 많은 깊이 있는 신호 확보

z-resolution를 개선하고 광학적 수차를 줄여 3D 샘플을 좀 더 정확하게 재구성할 수 있습니다.

더욱 선명하고 깨끗한 이미지 확보

더 나은 광 수집으로 더 짧은 노출 시간에 보다 밝은 세기의 이미지를 얻을 수 있으므로, 데이터의 품질 개선에 기여합니다.

자신감 있게 샘플 실행

MetaXpress 소프트웨어에는 물 컨트롤러 시스템의 시스템 상태를 표시하기 위해 통합된 사용자 직관적인 센서와 경보가 포함되어 샘플 실행 시 안심할 수 있습니다.

고처리량에서 3D 샘플 획득

수침 대물렌즈가 포함된 ImageXpress Micro Confocal 시스템은 획득 중에 물을 자동으로 보충하기 때문에 전체 마이크로플레이트 샘플을 고처리량으로 획득할 수 있습니다.

3D 구조의 보기 및 정량화 간소화

스페로이드, 미세 조직, 세포를 3D 매트릭스로, 그리고 z 평면의 스택으로 획득한 작은 유기체를 분석합니다. MetaXpress 3D 분석 모듈을 활용하여 부피, XYZ 위치, 인접 대물과의 거리, 직경, 깊이, 다양한 세기 측정 수치, 질감 또는 대물의 수를 평가할 수 있습니다.

이미지 획득 시간 단축

QuickID targeted acquisition images at low magnification to find objects of interest or rare events, then, automatically acquires high-magnification images with the required wavelengths, z-stack, or time points. 이 분석 프로세스는 선택한 표적을 고배율로 획득하여 획득 시간을 단축하고 이미징 저장 요구사항을 줄입니다.

^†고객이 제공한 샘플을 사용하여 개발 중 데이터 및 이미지를 획득했습니다. 결과에는 차이가 있을 수 있습니다.

더 많은 자료

Gain deeper insights into cellular 3D structures
Water immersion objectives for automated high-content imaging
3D image analysis and characterization of angiogenesis
Monitor mitochondria dynamics and phenotype with HCI
Water immersion technology and high-content imaging: A closer look
Improve 3D acquisition and analysis with water immersion objectives

Gain deeper insights into cellular 3D structures

Gain deeper insights into cellular 3D structures with water immersion objectives for high-content imaging. With the increasing interest in using three-dimensional (3D) cultures for assay development and phenotypic screening for a range of cellular models, water immersion objectives are essential for capturing more data at greater depths in 3D structures, such as spheroids and thick tissues.

더 알아보기

Water immersion objectives for automated high-content imaging

Water immersion objectives for automated high-content imaging to improve precision and quality of complex biological assays determining if water immersion objectives, used to improve image quality in complex biological assays, could be used in a high-throughput environment.

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3D image analysis and characterization of angiogenesis

Organ-on-a-chip model 에서 혈관 형성 후 3D Imaging 분석 및 특성분석

Visualize angiogenic sprouting and 3D reconstitution of structures
Perform quantitative assessment of angiogenesis, including number of sprouts, total volume, and average intensity
Generate physiologically-relevant results using the OrganoPlate^® platform

응용 분야 노트 읽기

Monitor mitochondria dynamics and phenotype with HCI

High-content imaging으로 미토콘드리아 동역학 및 표현형 모니터링

Achieve better visualization of changes in mitochondrial shape and structure
Quantify and measure phenotypic changes in a more efficient and precise manner
Understand mechanisms of disease and assess compound toxicity in a variety of cell models

응용 분야 노트 읽기

Water immersion technology and high-content imaging: A closer look

Three-dimensional (3D) cellular assays have emerged as a valuable tool in drug discovery and biological research, as they closely mimic in vivo environments and are shown to generate more physiologically relevant data than many 2D assays. With the increased utilization of 3D cultures for phenotypic screening and toxicity assessment, there is a growing need to optimize assay sensitivity, resolution, and throughput.

더 알아보기

Improve 3D acquisition and analysis with water immersion objectives

Sebastian Peck is a senior product manager for cellular imaging at Molecular Devices, one of the world's leading providers of high-performance bioanalytical measurement systems, software, and consumables for life science research, pharmaceutical, and biotherapeutic development.

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