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Organoid Innovation Center

Modular, automated 3D cell culture and image analysis lab enables customers to streamline and scale organoid interrogation, advancing drug discovery research

Automated 3D cell culture and image analysis lab streamlines and scales complex biology research

The new Organoid Innovation Center at Molecular Devices combines cutting-edge technologies with novel 3D biology methods to address key challenges of scaling complex 3D biology.

The collaborative space brings customers and researchers into the lab to test automated workflows for organoid culturing and screening, with guidance from in-house scientists.

An end-to-end solution standardizes the organoid development process with cell culture, treatment, and incubation, through to imaging, analysis, and data processing, delivering consistent, unbiased, and biologically-relevant results at scale.

Quickly adopt innovative, 3D biological methods and technologies for drug discovery

The center expands beyond imaging to demonstrate a fully-integrated solution that addresses the challenges associated with every step in the sample prep-to-report pipeline for assays performed on complex 3D biological models.

The Organoid Innovation Center showcases cutting-edge instruments that work harmoniously together for autonomous, long-term, live cell 2D and 3D cell culture growth and monitoring with intelligent label-free imaging. This integrated workflow provides quality control alerts and readiness, 3D organoid screening, and deep learning image analysis that reveals hidden patterns other technologies miss.

 

Organoid screening workcell at Molecular Devices Organoid Innovation Center

See the power and flexibility of an automated and customizable high-throughput screening solution

With intuitive scheduling software researchers can control the 3D workflow remotely, tracking the cell journey from single cell to differentiated organoid, along the way. Cell culture and incubation is streamlined with an automated incubator and collaborative robot that maintains culture consistency. Media exchange for culture maintenance is standardized and streamlined with automated liquid handling, minimizing manual intervention. 3D model development can be monitored over time with label-free imaging to assess assay readiness – and with real-time feedback, scheduling of automated compound addition and treatment is standardized.

실험과정

  • Step 1) 2d Pre-culture – Organoids are pre-cultured from iPSC-derived or primary cells (intestine, lung, or brain) 
  • 2단계) Developing 3D Organoids – Cells are transferred to 24-well plates then placed in an incubator to promote cell growth and differentiation into specific tissue in 3D
  • 3단계) Organoid Culture – Organoid culture process require multiple steps with different media exchanges
  • Step 4) Monitor organoid growth & development – Organoid are monitored and characterized for complex analysis of tissue structure and differentiation
  • 5단계) Confocal imaging and 3D analysis – The visualization and characterization of multiple quantitative descriptors used for studying disease phenotypes and compound effects
  • Our new ImageXpress Confocal HT.ai High-Content Imaging system, designed for 3D imaging. This system offers eight high-powered laser excitation channels and automated water immersion objectives that boosts signal and assay sensitivity without sacrificing speed.  Spinning disk confocal technology with five pinhole geometry options reduces haze from out of focus light for deeper organoid penetration and improved axial resolution. For analysis of complex 3D biology, IN Carta Image Analysis Software provides a streamlined workflow with powerful deep-learning based segmentation, machine learning-based classification, and 3D volumetric analysis.

High-performance 3D customizations

 

Molecular Devices can successfully tailor the system to include customized software and hardware including the features described herein, as well as integration of other lab components such as incubators, liquid handlers, and robotics for a fully automated workcell. 생명과학 분야에서 30년 이상의 경험과 더불어, 품질이 우수한 제품을 납품하고 전 세계 지원 서비스를 제공하고 있으므로 신뢰하실 수 있습니다.

판매는 www.moleculardevices.com/custom-products-purchase-terms에서 확인 가능한 당사의 맞춤형 제품 구매 약관에 따름

 

Lung organoid cell image gallery

응용 분야 및 assay

The Organoid Innovation Center builds on Molecular Devices 35 years of experience delivering high-performance life science technology to customers for improved drug development, biotechnology research, and clone screening workflows.

Learn more about our industry leading 3D biology and automated high-content imaging applications:

  • 3D Biology Articles

    3D Biology Articles

    Curated 3D biology articles from Molecular Devices subject matter experts, featured in trade publications like BioTechniques and Genetic Engineering & Biotechnology News.

    3D Cancer Cell Research

    3D Cancer Cell Research

    암은 세포가 정상 한계치 이상으로 생장 및 분열하고, 인접 조직을 칩입 및 파괴하며, 궁극적으로는 체내의 원발 부위로 전이하는 변화와 관련이 있습니다. 암 스페로이드는 기존 2D 세포 배양에 비해 종양 행동을 훨씬 효과적으로 모방합니다. 이 3D Spheroid 모델은 가능성 있는 암 치료를 식별하는 환경 스크리닝에 성공적으로 이용되고 있습니다.

    암 연구자에게는 복잡하거나 잘 알려지지 않은 암세포와 세포 환경 간의 상호작용을 보다 용이하게 연구하고 치료적 중재 지점을 식별하기 위한 도구가 필요합니다.

    자세히 알아보기 

  • 3D Cell Model

    3d Cell Model

    3D cell cultures offer the advantage of closely recapitulating aspects of human tissues including the architecture, cell organization, cell-cell and cell-matrix interactions, and more physiologically-relevant diffusion characteristics. 3D Cell Assay를 활용하면 2D Cell Culture와 Animal Model 사이의 연구와 스크리닝, 번역 과정의 다양성 측면에서의 격차를 좁혀주면서 그 가치를 더해줍니다. 3D Model은 in vivo 환경의 중요한 Parameter를 재현하여, 인공조직의 개발이나, 줄기세포의 특성에대한 Unique Insight를 제시합니다.

    자세히 보기 

    Cell Painting

    Cell Painting

    Cell Painting is a high-content, multiplexed image-based assay used for cytological profiling. In a Cell Painting assay, up to six fluorescent dyes are used to label different components of the cell including the nucleus, the endoplasmic reticulum, mitochondria, cytoskeleton, the Golgi apparatus, and RNA. The goal is to “paint” as much of the cell as possible to capture a representative image of the whole cell.

    자세히 알아보기 

  • 고객 사례 소개

    Revolutionizing early drug discovery

    Bioneer uses the ImageXpress Micro Confocal for high-throughput imaging of 3D disease models

    면역 종양학 및 신경퇴행성 질환 모델링을 위한 초기 신약 개발의 혁신화: High content imaging of 3D disease models

    자세히 알아보기  

    Disease Modeling

    Disease Modeling

    In this webinar, in collaboration with MIMETAS, we present some of the latest developments in integrating organoid protocols with organ-on-a-chip technology, as well as advancements in the high-content imaging technologies that are enabling these advanced 3D applications. We show how a range of tissue models comprising complex co-cultures can be formed in a perfused system, using state-of-the-art stem cell and organoid protocols, and how such models can then be screened and analyzed within a single, integrated interface to dramatically reduce time to discovery.

  • Moving from 2D to 3D Cell Culture

    Moving from 2D to 3D Cell Culture

    There has been a recent shift toward using 3D cell models in drug discovery and disease modeling, as numerous studies show they better mimic the in vivo environment and provide more physiologically-relevant data than 2D models.

    Organoids

    Organoids

    Organoids are three-dimensional (3D) multi-cellular microtissues that are designed to closely mimic the complex structure and functionality of human organs. Organoids typically consist of a co-culture of cells which demonstrate a high order of self-assembly to allow for an even better representation of complex in vivo cell responses and interactions, as compared to traditional 2D cell cultures.

    자세히 알아보기 

  • 줄기세포 연구

    줄기세포 연구

    만능 줄기세포는 발달 생물학 연구에 사용되며 Organ 특정 세포의 근원으로 분화되며, 슬라이드나 멀티 Well 플레이트에서 Live Cell 또는 Fixed cell based assay에 사용할 수 있습니다. ImageXpress 시스템은 분화 추적에서부터, 품질 관리, 특정한 세포 유형의 기능 측정에 이르는 줄기세포 연구자의 모든 워크플로 단계에서 유용합니다.

    자세히 알아보기 

    독성 스크리닝

    독성 스크리닝

    Organoid models have increasingly gained popularity in biologic research and screening to recapitulate complexity of real tissues. To model the in vivo human lungs, we have cultured primary human lung epithelial cells under conditions that promote the formation of 3D structures recapitulating the morphological and functional characteristics of the airway.

    Scientific poster 다운로드

Resources for Organoid Innovation Center

동영상 및 웨비나

Organoid Innovation Center Walkthrough

Organoid Innovation Center Walkthrough

21세기의 질병 모델링: 3D Imaging에 사용하는 자동화 오가노이드 assay

신약 개발 및 질병 모델링을 위한 High-throughput, 오가노이드 유래 Organ-on-a-Chip 시스템

High-content assay의 3D 전환: 과학적 기회와 이미징의 도전과제

Getting started with imaging 3D cell models – all you need to know

High-Content Imaging을 이용하여 신약개발용 High-Throughput Organ-on-a-chip 개발

High-throughput의 Organ-on-a-Chip 플랫폼을 이용한 생리학적으로 유의미한 조직 모델

High-Throughput의 Organ-on-a-chip 플랫폼을 이용한 생리학적으로 유의미한 조직 모델

관련 제품 및 서비스