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8개의 필터 옵션은 간소화된 assay 설정 제공

EMax® Plus 마이크로플레이트 리더기는 엔트리 레벨 플랫폼으로 확실한 솔루션을 제공합니다. 8개의 필터를 통해 단백질 정량, cell viability 및 ELISA와 같은 가시광선 파장 흡수 측정이 가능합니다. 이 리더기는 평면 및 원형 96웰 마이크로플레이트 둘 다 측정할 수 있으며 각각의 판독 전에 자동 램프 보정으로 정확성을 보장합니다.

  • 다양한 응용 분야에 적용

    다양한 응용 분야에 적용

    8개의 필터는 ELISA와 bioassay, Bradford, Lowry, BCA 및 DC 단백질 assay 등의 단백질 정량, 포스파타아제 및 키나아제, cell viability와 같은 다양한 응용 분야에 적용할 수 있습니다.

  • Assay 설정 간소화

    Assay 설정 간소화

    SoftMax® Pro 소프트웨어는 사전 설정 프로토콜, 표준 데이터 감소 설정, 자동 데이터 복구, 결과 시각화 및 분석으로 assay 설정을 간소화합니다.

  • 옵션 맞춤화

    옵션 맞춤화

    운동 판독 일시정지 및 다시 시작을 위한 불연속 운동 기능, 일반 데이터 분석 기능을 위한 사전 정의 계산 옵션 및 손쉬운 데이터 내보내기와 같은 다양한 옵션을 맞춤화합니다.

EMax plus Microplate Reader 를 활용한 ELISA 실험과정

EMax plus Microplate Reader 와 MultiWash+ Microplate Washer 를 활용한 ELISA 실험과정

기능

  • 손쉬운 데이터 시각화

    강력한 커브 피팅 프로토콜 및 통계 분석 기능이 포함되어 그레이스케일 또는 컬러 맵 이미지, 3D 그래프, 운동 플롯 또는 반응 속도로 획득된 데이터를 손쉽게 시각화할 수 있습니다.

  • 컴팩트한 디자인

    좁은 설치 공간 및 로우 프로파일로 벤치 공간을 절약합니다.

EMax Plus 마이크로플레이트 리더기의 응용 분야

  • Absorbance

    Absorbance

    작동 방법, 측정 방법, 농도 계산에 사용될 수 있는 방법 등 흡광 검출에 대한 모든 것을 알아보십시오. ELISA, 핵산 및 단백질 정량, 그리고 미생물 생장 등 일반 흡광도 측정에 관련된 실험과 분석법에 대한 정보를 제공합니다.

    자세히 알아보기 

    ELISA

    Elisa

    ELISA는 Microplate 를 사용하여 특정 단백질의 양을 측정하는 데 사용되며 결과는 대부분의 경우 가시광선 파장 범위의 흡광도 측정을 통해 검출됩니다. Chemiluminescence 및 형광 ELISA 형식은 감도가 좋아 소량의 샘플의 경우에도 정확한 정량이 가능합니다.

    자세히 알아보기 

  • 단백질 정량(Bradford, Lowry, BCA, DC)

    단백질 정량

    단백질 농도는 UV 분광 광도계에서 280nm 기준 흡광을 통해 직접, 또는 BCA 혹은 Bradford assay와 같은 측색 방법을 사용하여 간접적으로 측정할 수 있습니다. 흡광 정량은 추가 시약이 필요하지 않기 때문에 실행하기 쉽지만 측색 방법은 더 높은 감도를 제공하기 때문에 샘플의 가격이 높은 경우 흔히 선호되는 방법입니다. 두 가지 방법 모두 UV 가시광선 분광 광도계 또는 흡광 마이크로플레이트 리더기에서 수행할 수 있습니다.

    Application Note 자세히 보기 

EMax Plus 마이크로플레이트 리더기의 사양 및 옵션

 

*사용 가능 시 최저 설정 및 속도 판독 사용.

EMax Plus 마이크로플레이트 리더기 자료

프레젠테이션
동영상 및 웨비나
EMax plus Microplate Reader 를 활용한 ELISA 실험과정

EMax plus Microplate Reader 와 MultiWash+ Microplate Washer 를 활용한 ELISA 실험과정

  • Citation
    Dated: Dec 14, 2020
    Publication Name: Brain, Behavior, and Immunity

    Cytokine Dysregulation Associated with Exam Stress in Healthy Medical Students

    The mechanisms of stress-related immune alterations have not been fully elucidated. Cell-mediated immune responses as well as antibody and certain cytokines are reported as being suppressed during times of high stress. However, the role of suppression vs dysregulation has not been established in human stress models. The effect of exam stress on… View more

    The mechanisms of stress-related immune alterations have not been fully elucidated. Cell-mediated immune responses as well as antibody and certain cytokines are reported as being suppressed during times of high stress. However, the role of suppression vs dysregulation has not been established in human stress models. The effect of exam stress on regulatory cytokines in 16 healthy medical students was assessed by measuring type-1 (IFN-γ) and type-2 (IL-10) cytokines from 72-h PHA/PMA-stimulated PBMC 4 weeks before and 48 h after exams. Results demonstrated decreased IFN-γ accompanied by increased IL-10 during exam stress that resulted in a decreased IFN-γ:IL-10 ratio. There was a significant correlation between the cytokine response to PHA/PMA and number and subjective adjustment to daily hassles. Additionally, students who reported greater levels of loneliness also reported greater numbers of and poorer subjective adjustment to hassles. The differences were consistent in both males and females but did not correlate with AM cortisol levels. Additionally, when individuals were grouped into high vs low preexam hassle levels, the type-1/type-2 shift in the IFN-γ:IL-10 ratio occurred in the low hassles group only. These data suggest that psychologically stressful situations shift type-1/type-2 cytokine balance toward type-2 and result in an immune dysregulation rather than overall immunosuppression. This may partially explain the increased incidence of type-2-mediated conditions such as increased viral infections, latent viral expression, allergic/asthmatic reactions, and autoimmunity reported during periods of high stress.

    Contributors: Gailen D.Marshall Jr, Sandeep K. Agarwal, Camille Lloyd, Lorenzo Cohen, Evelyn M. Henninger, Gloria J. Morris  
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  • Citation
    Dated: Jun 07, 2008
    Publication Name: J. Microbiol. Biotechnol

    Investigation of the Antifungal Activity and Mechanism of Action of LMWS-Chitosan

    Chitosan, a cationic polysaccharide, has been widely used as a dietary supplement and in a variety of pharmacological and biomedical applications. The antifungal activity and mechanism of action of low molecular weight water-soluble chitosan (LMWS-chitosan) were studied in fungal cells and vesicles containing various compositions of fungal lipids… View more

    Chitosan, a cationic polysaccharide, has been widely used as a dietary supplement and in a variety of pharmacological and biomedical applications. The antifungal activity and mechanism of action of low molecular weight water-soluble chitosan (LMWS-chitosan) were studied in fungal cells and vesicles containing various compositions of fungal lipids. LMWS-chitosan showed strong antifungal activity against various pathogenic yeasts and hyphae-forming fungi but no hemolytic activity or cytotoxicity against mammalian cells. The degree of calcein leakage was assessed on the basis of lipid composition (PC/CH; 10:1, w/w). Our result showing that LMWS-chitosan interacts with liposomes demonstrated that chitosan induces leakage from zwitterionic lipid vesicles. Confocal microscopy revealed that LMWSchitosan was located in the plasma membrane. Finally, scanning electron microscopy revealed that LMWS-chitosan causes significant morphological changes on fungal surfaces. Its potent antibiotic activity suggests that LMWS-chitosan is an excellent candidate as a lead compound for the development of novel anti-infective agents

    Contributors: Park, Yoonkyung, Mi-Hyun Kim, Seong-Cheol Park, Hyeonsook Cheong, Mi-Kyeong Jang, Jae-Woon Nah, and Kyung-Soo Hahm  
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  • Citation
    Dated: Oct 01, 1995
    Publication Name: The Journal of Infectious Diseases

    Drug Cytotoxicity Assay for African Trypanosomes and Leishmania Species

    The trypanosomes and Leishmania species are parasitic protozoa that afflict millions of people throughout the world. If not treated, African trypanosomiasis and visceral leishmaniasis are fatal. The available drugs are severely limited by toxicity, marginal efficacy, the requirement for parenteral administration, and spreading drug resistance. In… View more

    The trypanosomes and Leishmania species are parasitic protozoa that afflict millions of people throughout the world. If not treated, African trypanosomiasis and visceral leishmaniasis are fatal. The available drugs are severely limited by toxicity, marginal efficacy, the requirement for parenteral administration, and spreading drug resistance. In this study, a spectrophotometric assay was developed and validated for measuring the cytotoxicity of test compounds against axenically cultured bloodstream-form Trypanosoma brucei (African trypanosomes) and promastigotes of Leishmania donovani. Enzymatic hydrolysis of p-nitrophenyl phosphate, monitored by a microtiter plate reader, is a reliable surrogate for parasite cell counts. The assay is simple, inexpensive, and highly reproducible. The coefficient of variation for EC50 values is <10% for determinations obtained over several months. This method permits the rapid screening of candidates for much-needed new drugs against these parasites.

    Contributors: Annette L. Bodley, Michael W. McGarry, Theresa A. Shapiro  
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EMax Plus 마이크로플레이트 리더기의 관련 제품 및 서비스