Molecular Devices Lab Notes

SLAS Europe 2022, hosted numerous sessions packed with the latest research on emerging topics as well as sessions and panel discussions focused on how to build, and succeed, in a career within the life sciences or biotech industries.

We were delighted to attend SLAS EU 2022 in Dublin this year. We would like to thank all of you for visiting our booth, and for the opportunity to showcase our market-leading solutions to the scientific community. At the conference, we had the opportunity to deliver a series of educational presentations, together with our partners — From new advancements in engineering next-generation organoids to developing an automated lab workflow for 3D biology encompassing culturing, monitoring and high-content imaging.

 

Table of Contents

  1. Automation of compound screening and high content imaging of 3D triple-negative breast cancer patient-derived tumoroids
  2. Automation of the organ-on a chip assay: automated culture, imaging and analysis of angiogenesis
  3. Structural Organization and Functional Analysis of Compound Responses in 3D human iPSC-derived Cardiac Tri-Culture Microtissues
  4. Assessing thrombin generation with the TECHNOTHROMBIN TGA kit on the SpectraMax i3x Multi-Mode Microplate Reader
  5. Evaluation of a fluorescent approach to implement monoclonality assurance using calcein-AM viability dye.
  6. Novel 3D Tissue Models, Imaging and Automation of Organ-On-A-Chip Assays.

 

1. Automation of compound screening and high content imaging of 3D triple-negative breast cancer patient-derived tumoroids

Authors: Oksana Sirenko | Molecular Devices LLC

3D triple-negative breast cancer patient-derived tumoroids

Finding efficient drug combinations to treat cancer patients is critical for therapy success, especially for rare types of tumors that resist traditional therapy. Triple negative breast cancer is a clinically aggressive subtype, with high rates of metastasis, recurrence, and drug resistance, and no clinically approved small molecule targeted therapies. There is a critical need to develop methods for efficient testing drug efficacy in patient-derived tumor samples to discover new therapeutic targets. Patient-derived 3D cancer models are highly valuable tools for cancer research and drug development. However, the complexity of performing 3D assays remains a hurdle for adopting these methods for compound screening. In the present study we describe automation of imaging, analysis, and cell culture methods which enables scaling up complex 3D cell-based assays and compound screening.

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2. Automation of the organ-on a chip assay: automated culture, imaging and analysis of angiogenesis

Authors: Angeline Lim, Oksana Sirenko | Molecular Devices LLC  
Arthur Stok, Matthew Delport | Mimetas  
Francis Enane | Beckman Coulter Life Sciences

Automated culture, imaging and analysis of angiogenesis

There is a critical need for biological model systems that better resemble human biology in drug discovery. The OrganoPlate® was developed as an organ-on-a-chip platform allowing the formation of three-dimensional (3D), microfluidic-based, long-term cultures of live cells. However, the complexity of 3D models remains a hurdle for wide adoption in research and drug screening. Automation of the cell culture, assays, and analysis can provide the tools necessary to facilitate and scale up the use of organo-on chip systems.

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3. Structural Organization and Functional Analysis of Compound Responses in 3D human iPSC-derived Cardiac Tri-Culture Microtissues

Authors: Simon Lydford1, Zhisong Tong1, Carole Crittenden1, Angeline Lim1, Sarah Himmerich2, Cara Rieger2, Ravi Vaidyanathan2, Coby Carlson2, and Oksana Sirenko1  
Molecular Devices1  
FUJIFILM Cellular Dynamics, Inc.2

3D human iPSC-derived Cardiac Tri-Culture Microtissues

The human heart is a complex organ providing highly regulated processes of moving blood through the body. The adult human ventricle is comprised of cardiomyocytes, endothelial cells, fibroblasts and other supporting cell types. Though cardiomyocytes make up 75% of the total volume of human ventricle, it only constitutes 50% of the total cell number. Recent publications show that tri-cellular co-culture microtissues of cardiomyocytes, endothelial cells and cardiac fibroblasts all derived from human iPSC enhance the maturation and functional activity of cells compared to 2D cardiomyocytes and thus more closely mimics actual heart physiology. In this study, we used a tri-culture model created by mixing iPSC-derived cardiac cells with primary adult fibroblasts and iPSC-derived endothelial cells at 70:20:10 ratio in ultra-low attachment (ULA) plates directly from thaw.

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4. Assessing thrombin generation with the TECHNOTHROMBIN TGA kit on the SpectraMax i3x Multi-Mode Microplate Reader

Authors: Rebecca Kilmartin4, Cathleen Salomo3, Cathy Olsen2and Lieselotte Wagner1  
1Technoclone Herstellung von Diagnostika und Arzneimitteln GmbH, 2Molecular Devices, LLC, 3Molecular Devices (Germany) GmbH and 4Molecular Devices (UK) Ltd

TECHNOTHROMBIN TGA kit on the SpectraMax i3x Multi-Mode Microplate Reader

Assessing the generation of thrombin in a plasma sample allows one to better understand coagulation mechanisms. To meet the needs of researchers to determine the time-dependent changes in thrombin concentrations on a flexible platform, Technoclone has developed a plate reader-compatible kinetic assay format, the TECHNOTHROMBIN® TGA kit, using a fluorogenic substrate. This poster demonstrates that the combination of the SpectraMax i3x reader and TECHNOTHROMBIN TGA kit offers an ideal platform to perform thrombin generation assays with good precision for research use.

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5. Evaluation of a fluorescent approach to implement monoclonality assurance using calcein-AM viability dye.

Authors: Carola Mancini, PhD, Sarmad al-Bassam, PhD  
Molecular Devices, LLC, Munich, Germany

Monoclonality assurance using calcein-AM viability dye

Assessing monoclonality is key to the establishment of a cell line, and evidence of monoclonality is required by regulatory agencies to get a biopharmaceutical drug to the marketplace. Here, we demonstrate an optimized workflow using the fluorescence reagent, calcein-AM (CAM), in conjunction with a fluorescence-capable CloneSelect™ Imager (CSI-FL) that shows similar viability to label-free conditions while simultaneously providing high assurance of clonality. In this workflow, we determine a concentration of CAM that is ideal for detection of single cells on a CloneSelect™ Imager-FL while minimizing cytotoxic effects on clonal outgrowth. We describe guidelines for establishing an optimal concentration of viability dye for different cell types.

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6. Novel 3D Tissue Models, Imaging and Automation of Organ-On-A-Chip Assays.

Authors: Oksana Sirenko - Molecular Devices, LLC,  
Chiwan Chiang - MIMETAS

Novel 3D Tissue Models, Imaging and Automation of Organ-On-A-Chip Assays

To enhance drug discovery & development, there is a critical need for complex biological model systems that better resemble human biology. In this tutorial, we provide an overview of complex 3D tissue models & assays, such as immune cell migration, and an introduction to high content imaging and image analysis methods, showing how you can get powerful new insights from complex biological models.

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